10 Questions You Should Know about Pipettes for Cell Culture

## 10 Questions You Should Know about Pipettes for Cell Culture.

1. What is a pipette?

2. Why is it important to use accurate pipettes in cell culture?

3. What are the different types of pipettes used in cell culture?

4. How do you calibrate a pipette?

5. How often should pipettes be calibrated?

6. What is the difference between air-displacement and positive-displacement pipettes?

7. How should pipettes be maintained and cleaned?

8. Can pipettes be used for different volumes?

9. What are some common mistakes to avoid when using a pipette?

10. Where can I purchase high-quality pipettes for cell culturepipettes for cell culture?

## Answers.

1. A pipette is a laboratory instrument used to accurately measure and transfer small volumes of liquid.

2. Accurate pipetting is crucial in cell culture as small errors can affect cell viability and experimental results.

3. The main types of pipettes used in cell culture include air-displacement pipettes and positive-displacement pipettes.

4. Pipettes should be calibrated using a gravimetric method, where the actual weight of liquid dispensed is measured and compared to the desired volume.

5. Pipettes should be calibrated at least once a year, or more frequently if they are used frequently or dropped.

6. Air-displacement pipettes are suitable for aqueous solutions, while positive-displacement pipettes are used for viscous or volatile liquids.

7. Pipettes should be regularly maintained by cleaning them with appropriate solutions and storing them properly when not in use.

8. Most pipettes can be used for a range of volumes by adjusting the volume setting.

9. Common mistakes to avoid when using a pipette include not priming the pipette, using the wrong tip size, and not holding the pipette vertically.

10. High-quality pipettes for cell culture can be purchased from laboratory suppliers, such as VWR, Thermo Fisher Scientific, or Gilson.

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