10 Things You Must Know About 96-Well Plates for ELISA

10 Things You Must Know About 96-Well Plates for ELISA.

1. What are 96-well plates?

96-well plates are plastic or glass plates that contain 96 wells in a 8x12 matrix format, with each well having a capacity of approximately 300 μL.

2. What are they used for?

96-well plates are commonly used in laboratories for high-throughput screening assays and experiments, such as ELISA (enzyme-linked immunosorbent assay), PCR (polymerase chain reaction), and cell culture.

3. What materials can they be made of?

96-well plates can be made of glass or various types of plastic, such as polystyrene, polycarbonate, and cycloolefin copolymer. The type of material used depends on the specific application.

4. What are the different types of 96-well plates?

There are several types of 96-well plates available, including flat bottom, round bottom, V-bottom, and U-bottom plates, as well as plates with different well volumes and surface treatments.

5. What is ELISA?

ELISA is a common immunoassay technique used to detect and quantify proteins, peptides, antibodies, and hormones in biological samples.

6. How are 96-well plates used in ELISA?

In ELISA, 96-well plates are coated with an antigen or capture antibody, followed by the addition of a sample containing the target analyte. The plate is then incubated with a detection antibody and a substrate that produces a signal, which is detected by a plate reader.

7. What are the advantages of using 96-well plates for ELISA?

Using 96-well plates for ELISA allows for high-throughput screening of multiple samples simultaneously, reducing the time and cost of the assay. Additionally, the plates are compatible with automated plate readers and liquid handling systems.

8. How should 96-well plates be stored?

96-well plates should be stored in a dry, cool place, away from direct sunlight or strong oxidizing agents. They should also be protected from physical damage and contamination.

9. How should 96-well plates be handled?

When handling 96-well plates, it is important to avoid touching the bottom of the wells to prevent contamination. Additionally, the plates should be carefully labeled, and the plate orientation should be maintained throughout the assay.

10. Are 96-well plates reusable?

96-well plates are generally not reusable, as they are typically made of plastic or disposable glass. However, some glass plates can be cleaned and sterilized for reuse, depending on the application.

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