The concept of a SARS-CoV-2 / COVID-19 rapid test

       Diagnostic and screening tests that combine simplicity, speed, and reliable, clear readouts are essential tools in clinical settings. Specific analytes in a sample, such as pathogens or biomarkers, can be detected by lateral flow assay. Lateral flow assays are generally very easy to use and provide rapid information as to the presence or absence of a given analyte in a biological sample.

       Most of COVID-19 rapid tests utilize sandwich immunoassay technology, which detects SARS-CoV-2 particles in samples by capturing viral antigen between a free labeled antibody and a fixed second antibody that each recognize different regions of the antigen. On the other hand, a rapid anti-virus IgM/IgG test uses a similar approach to detect human IgM and IgG antibodies that are reactive to SARS-CoV-2. This provides crucial information related to whether a patient has been exposed to the virus and where they are temporally in the clinical course of the disease. Please see Table 1 and Figure 1 for specific details.

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Table 1. COVID-19 Rapid Tests

Assay Type Detection Target Reagents Used in the Test Clinical Significance Rapid virus antigen test SARS-CoV-2 nucleocapsid/spike protein  Nucleocapsid protein antibodies/
spike protein antibodies Ongoing viral production Rapid anti-virus IgM/IgG test Anti-SARS-CoV-2 IgM and IgG antibodies (1) Anti-human IgM and anti-human IgG antibodies

(2) Labeled viral antigen (spike/nucleocapsid protein)

Assessment of patient&#;s immune response to past or ongoing viral infection

 

Figure 1: Relationship between the amount/type of antibody generated against SARS-CoV-2 and the clinical stage of COVID-19 disease.
(In some young people or special conditions, IgM may appear on the fourth day.)

How do COVID-19 Rapid Tests Work?

COVID-19 rapid test strips contain a control line to confirm the test is working properly along with one or two test lines. Analyte detection is qualitative and read visually. Below are links to two animated videos detailing the components inside the rapid test devices and how they interact with one another to produce the colored results.

1. SARS-CoV-2 rapid virus antigen test: Please click on link (https://youtu.be/PkczTPfIE20). Possible result readouts are shown in Figure 2 below.

 

Figure 2: Possible result readouts of a SARS-CoV-2 rapid virus antigen test.

Contact us to discuss your requirements of strep a rapid test. Our experienced sales team can help you identify the options that best suit your needs.

2. SARS-CoV-2 rapid anti-virus IgM/IgG test: Please click on link (https://youtu.be/bAHFylmV5D8). Possible result readouts are shown in Figure 3.

 

Figure 3: Possible result readouts of a SARS-CoV-2 rapid anti-virus IgM/IgG test.

GeneTex is proud to offer an extensive line of reagents to support the study of SARS-CoV-2/COVID-19. Please see Table 2, Table 3, and Table 4 below to view listings of GeneTex's antibodies, recombinant proteins, and cell pellet blocks available now to accelerate your SARS-CoV-2/COVID-19 research.

 

Table 2. Antibodies for SARS-CoV-2 / COVID-19 Research

Clonality Cat. No. Product Name Host Applications Recombinant GTX SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] Rabbit WB, ICC/IF, ELISA, sELISA GTX SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] Rabbit WB, ICC/IF, ELISA GTX SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] Rabbit WB, ICC/IF, ELISA GTX SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] Rabbit WB, ICC/IF, ELISA, sELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] Rabbit WB, ICC/IF, ELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] Rabbit WB, ICC/IF, ELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] Rabbit WB, ICC/IF, ELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL] Rabbit WB, ELISA, sELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] Rabbit WB, ELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] Rabbit WB, ELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] Rabbit WB, ELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL] Rabbit WB, ELISA GTX SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR] Human ELISA, Neutralizing/Blocking GTX SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR-RB] Rabbit ELISA, Neutralizing/Blocking Monoclonal GTX SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] Mouse WB, ICC/IF, IHC-P, FACS, IP, ELISA, sELISA GTX SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] Mouse WB, ICC/IF, IHC-P, IP, ELISA, sELISA GTX SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] Mouse WB, ICC/IF GTX SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] Mouse WB Polyclonal GTX SARS-CoV-2 (COVID-19) Spike S1 antibody Rabbit WB, ICC/IF GTX SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody Rabbit WB, ICC/IF, ELISA, sELISA GTX SARS-CoV-2 (COVID-19) Spike RBD antibody Rabbit WB, ICC/IF GTX SARS-CoV-2 (COVID-19) Spike antibody Rabbit WB, ICC/IF, IHC-P, ELISA GTX SARS-CoV-2 (COVID-19) Spike antibody Rabbit WB, ICC/IF, ELISA, sELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody Rabbit WB, ICC/IF, IHC-P, IP, ELISA, sELISA GTX SARS-CoV-2 (COVID-19) nucleocapsid antibody Rabbit WB, ICC/IF, IHC-P, ELISA, sELISA GTX ACE2 antibody [N1N2], N-term Rabbit WB, IHC-P, FACS, ELISA GTX TMPRSS2 antibody [N2C3] Rabbit WB, IHC-P

 

Table 3. Recombinant Proteins for SARS-CoV-2 / COVID-19 Research

Cat. No. Product Name GTX-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) GTX-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) GTX-pro SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag GTX-pro SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) GTX-pro SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag GTX-pro SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag GTX-pro SARS-CoV-2 (COVID-19) Envelope protein, His and Avi tag GTX-pro SARS-CoV-2 (COVID-19) 3C-like Proteinase protein, His tag GTX-pro SARS-CoV-2 (COVID-19) 3C-like Proteinase protein, His and Avi tag

 

Table 4. Cell Pellet Blocks for SARS-CoV-2 / COVID-19 Research

Cat. No. Product Name GTX SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet block GTX SARS-CoV-2 (COVID-19) Spike S1 FFPE 293T cell pellet block GTX SARS-CoV-2 (COVID-19) Spike S2 FFPE 293T cell pellet block GTX SARS-CoV-2 (COVID-19) Nucleocapsid FFPE 293T cell pellet Block GTX SARS-CoV-2 (COVID-19) Membrane FFPE 293T cell pellet Block GTX SARS-CoV-2 (COVID-19) Envelope FFPE 293T cell pellet Block